Use of ChIP-qPCR to Study the Crosstalk Between HIF and NF-kappa B Signaling in Hypoxia and Normoxia

Shakir, Dilem, Batie, Michael ORCID: 0000-0002-7508-6641 and Rocha, Sonia (2021) Use of ChIP-qPCR to Study the Crosstalk Between HIF and NF-kappa B Signaling in Hypoxia and Normoxia. NF-(KAPPA)B TRANSCRIPTION FACTORS, 2366. pp. 255-265.

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Official URL: http://dx.doi.org/10.1007/978-1-0716-1669-7_15

Abstract

Hypoxia and inflammation are intensely connected in a functional crosstalk. Within this crosstalk, two major transcription factors take center stage: HIF and NF-κB. To investigate transcription factor function, an important aspect is its ability to bind DNA. The most appropriate method to study this property in cells is the use of chromatin immunoprecipitation followed by qPCR and/or next generation sequencing. This allows identification of potentially directly regulated genes as well as enhancer regions. Here we describe the ChIP-qPCR method in detail, including key aspects important for the success of the technique.

Item Type:	Article
Uncontrolled Keywords:	ChIP, HIF, Hypoxia, Inflammation, NF-kappaB, qPCR
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	29 Sep 2023 09:42
Last Modified:	29 Sep 2023 09:42
DOI:	10.1007/978-1-0716-1669-7_15
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3173210