Aye, Cho-Cho, Hammond, Dean E, Rodriguez-Cuenca, Sergio, Doherty, Mary K, Whitfield, Phillip D, Phelan, Marie M, Yang, Chenjing, Perez-Perez, Rafael, Li, Xiaoxin, Diaz-Ramos, Angels et al (show 6 more authors)
(2023)
CBL/CAP Is Essential for Mitochondria Respiration Complex I Assembly and Bioenergetics Efficiency in Muscle Cells.
International journal of molecular sciences, 24 (4).
p. 3399.
Abstract
CBL is rapidly phosphorylated upon insulin receptor activation. Mice whole body CBL depletion improved insulin sensitivity and glucose clearance; however, the precise mechanisms remain unknown. We depleted either CBL or its associated protein SORBS1/CAP independently in myocytes and assessed mitochondrial function and metabolism compared to control cells. CBL- and CAP-depleted cells showed increased mitochondrial mass with greater proton leak. Mitochondrial respiratory complex I activity and assembly into respirasomes were reduced. Proteome profiling revealed alterations in proteins involved in glycolysis and fatty acid degradation. Our findings demonstrate CBL/CAP pathway couples insulin signaling to efficient mitochondrial respiratory function and metabolism in muscle.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Mitochondria, Mitochondria, Muscle, Muscle Cells, Animals, Mice, Insulin Resistance, Insulin, Cell Respiration, Energy Metabolism, Proto-Oncogene Proteins c-cbl |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 14 Dec 2023 09:18 |
| Last Modified: | 14 Dec 2023 09:29 |
| DOI: | 10.3390/ijms24043399 |
| Open Access URL: | https://doi.org/10.3390/ijms24043399 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3177381 |
Dimensions
Dimensions
