Obolski, Uri 
ORCID: 0000-0001-7594-9745, Swarthout, Todd D ORCID: 0000-0001-5285-7039, Kalizang'oma, Akuzike, Mwalukomo, Thandie S, Chan, Jia Mun ORCID: 0000-0003-4223-7527, Weight, Caroline M ORCID: 0000-0001-6235-550X, Brown, Comfort, Cave, Rory, Cornick, Jen, Kamng'ona, Arox Wadson ORCID: 0000-0002-0841-7586 et al (show 8 more authors)
(2023)
The metabolic, virulence and antimicrobial resistance profiles of colonising Streptococcus pneumoniae shift after PCV13 introduction in urban Malawi.
Nature communications, 14 (1).
7477-.
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The metabolic, virulence and antimicrobial resistance profiles of colonising Streptococcus pneumoniae shift after PCV13 intr.pdf - Open Access published version Download (3MB) | Preview |
Abstract
Streptococcus pneumoniae causes substantial mortality among children under 5-years-old worldwide. Polysaccharide conjugate vaccines (PCVs) are highly effective at reducing vaccine serotype disease, but emergence of non-vaccine serotypes and persistent nasopharyngeal carriage threaten this success. We investigated the hypothesis that following vaccine, adapted pneumococcal genotypes emerge with the potential for vaccine escape. We genome sequenced 2804 penumococcal isolates, collected 4-8 years after introduction of PCV13 in Blantyre, Malawi. We developed a pipeline to cluster the pneumococcal population based on metabolic core genes into "Metabolic genotypes" (MTs). We show that S. pneumoniae population genetics are characterised by emergence of MTs with distinct virulence and antimicrobial resistance (AMR) profiles. Preliminary in vitro and murine experiments revealed that representative isolates from emerging MTs differed in growth, haemolytic, epithelial infection, and murine colonisation characteristics. Our results suggest that in the context of PCV13 introduction, pneumococcal population dynamics had shifted, a phenomenon that could further undermine vaccine control and promote spread of AMR.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Nasopharynx, Animals, Humans, Mice, Streptococcus pneumoniae, Pneumococcal Infections, Pneumococcal Vaccines, Anti-Bacterial Agents, Carrier State, Drug Resistance, Bacterial, Virulence, Child, Child, Preschool, Infant, Malawi, Serogroup |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 21 Dec 2023 16:46 |
| Last Modified: | 21 Dec 2023 16:46 |
| DOI: | 10.1038/s41467-023-43160-y |
| Open Access URL: | https://doi.org/10.1038/s41467-023-43160-y |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3177616 |
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