SARS-CoV-2 NSP12 associates with TRiC and the P323L substitution acts as a host adaption.

Alruwaili, Muhannad, Armstrong, Stuart ORCID: 0000-0002-3862-1801, Prince, Tessa ORCID: 0000-0002-8796-2629, Erdmann, Maximillian, Matthews, David A, Luu, Lisa ORCID: 0000-0002-7748-5961, Davidson, Andrew, Aljabr, Waleed and Hiscox, Julian A ORCID: 0000-0002-6582-0275 (2023) SARS-CoV-2 NSP12 associates with TRiC and the P323L substitution acts as a host adaption. Journal of virology, 97 (11). e0042423-e0042423.

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Official URL: http://dx.doi.org/10.1128/jvi.00424-23

Abstract

<h4>Importance</h4>SARS-CoV-2 has caused a worldwide health and economic crisis. During the course of the pandemic, genetic changes occurred in the virus, which have resulted in new properties of the virus-particularly around gains in transmission and the ability to partially evade either natural or vaccine-acquired immunity. Some of these viruses have been labeled Variants of Concern (VoCs). At the root of all VoCs are two mutations, one in the viral spike protein that has been very well characterized and the other in the virus polymerase (NSP12). This is the viral protein responsible for replicating the genome. We show that NSP12 associates with host cell proteins that act as a scaffold to facilitate the function of this protein. Furthermore, we found that different variants of NSP12 interact with host cell proteins in subtle and different ways, which affect function.

Item Type:	Article
Uncontrolled Keywords:	Cytosol, Humans, Mutation, Adaptive Immunity, MARVEL Domain Containing 2 Protein, Spike Glycoprotein, Coronavirus, COVID-19, SARS-CoV-2, Coronavirus RNA-Dependent RNA Polymerase
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	12 Jan 2024 08:57
Last Modified:	12 Jan 2024 08:58
DOI:	10.1128/jvi.00424-23
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3177806