The Structure of the talin head reveals a novel extended conformation of the FERM domain.

Elliott, Paul R ORCID: 0000-0002-7641-2103, Goult, Benjamin T ORCID: 0000-0002-3438-2807, Kopp, Petra M, Bate, Neil, Grossmann, J Günter, Roberts, Gordon CK ORCID: 0000-0001-6200-1373, Critchley, David R and Barsukov, Igor L ORCID: 0000-0003-4406-9803 (2010) The Structure of the talin head reveals a novel extended conformation of the FERM domain. Structure (London, England : 1993), 18 (10). pp. 1289-1299.

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Official URL: http://dx.doi.org/10.1016/j.str.2010.07.011

Abstract

FERM domains are found in a diverse superfamily of signaling and adaptor proteins at membrane interfaces. They typically consist of three separately folded domains (F1, F2, F3) in a compact cloverleaf structure. The crystal structure of the N-terminal head of the integrin-associated cytoskeletal protein talin reported here reveals a novel FERM domain with a linear domain arrangement, plus an additional domain F0 packed against F1. While F3 binds β-integrin tails, basic residues in F1 and F2 are required for membrane association and for integrin activation. We show that these same residues are also required for cell spreading and focal adhesion assembly in cells. We suggest that the extended conformation of the talin head allows simultaneous binding to integrins via F3 and to PtdIns(4,5)P2-enriched microdomains via basic residues distributed along one surface of the talin head, and that these multiple interactions are required to stabilize integrins in the activated state.

Item Type:	Article
Additional Information:	## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords:	Cell Line, Animals, Humans, Mice, Talin, Green Fluorescent Proteins, Integrin beta Chains, Microscopy, Fluorescence, Crystallization, X-Ray Diffraction, RNA Interference, Binding Sites, Protein Conformation, Protein Structure, Tertiary, Protein Binding, Mutation, Models, Molecular, Scattering, Small Angle
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	13 Mar 2024 09:34
Last Modified:	13 Mar 2024 09:34
DOI:	10.1016/j.str.2010.07.011
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3179349