<i>In vitro</i> evaluation of physicochemical-dependent effects of polymeric nanoparticles on their cellular uptake and co-localization using pulmonary calu-3 cell lines.

Osman, Nashwa, Curley, Paul ORCID: 0000-0003-4596-2708, Box, Helen, Liptrott, Neill ORCID: 0000-0002-5980-8966, Sexton, Darren ORCID: 0000-0003-3344-3150 and Saleem, Imran ORCID: 0000-0003-2382-6668 (2024) <i>In vitro</i> evaluation of physicochemical-dependent effects of polymeric nanoparticles on their cellular uptake and co-localization using pulmonary calu-3 cell lines. Drug development and industrial pharmacy, 50 (4). pp. 376-386.

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Abstract

<h4>Objective</h4>The study evaluated physicochemical properties of eight different polymeric nanoparticles (NPs) and their interaction with lung barrier and their suitability for pulmonary drug delivery.<h4>Methods</h4>Eight physiochemically different NPs were fabricated from Poly lactic-co-glycolic acid (PLGA, PL) and Poly glycerol adipate-co-ω-pentadecalactone (PGA-co-PDL, PG) <i>via</i> emulsification-solvent evaporation. Pulmonary barrier integrity was investigated <i>in vitro</i> using Calu-3 under air-liquid interface. NPs internalization was investigated using a group of pharmacological inhibitors with subsequent microscopic visual confirmation.<h4>Results</h4>Eight NPs were successfully formulated from two polymers using emulsion-solvent evaporation; 200, 500 and 800 nm, negatively-charged and positively-charged. All different NPs did not alter tight junctions and PG NPs showed similar behavior to PL NPs, indicating its suitability for pulmonary drug delivery. Active endocytosis uptake mechanisms with physicochemical dependent manner were observed. In addition, NPs internalization and co-localization with lysosomes were visually confirmed indicating their vesicular transport.<h4>Conclusion</h4>PG and PL NPs had shown no or low harmful effects on the barrier integrity, and with effective internalization and vesicular transport, thus, prospectively can be designed for pulmonary delivery applications.

Item Type:	Article
Uncontrolled Keywords:	Lung, Cell Line, Lactic Acid, Polyglycolic Acid, Drug Carriers, Solvents, Nanoparticles, Polylactic Acid-Polyglycolic Acid Copolymer
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	03 Apr 2024 14:22
Last Modified:	26 Apr 2024 21:09
DOI:	10.1080/03639045.2024.2332889
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3180039