Investigating the role of CFTR in human and mouse myometrium.

Prendergast, Clodagh, Wray, Susan, Dungate, Daniella, Martin, Christine, Vaida, Andra, Brook, Elizabeth, Chioma, Cecilia Ani and Wallace, Helen ORCID: 0000-0003-2254-8301 (2024) Investigating the role of CFTR in human and mouse myometrium. Current research in physiology, 7. p. 100122.

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Official URL: http://dx.doi.org/10.1016/j.crphys.2024.100122

Abstract

<h4>Background</h4>Abnormal cystic fibrosis transmembrane conductance regulator (CFTR) function in cystic fibrosis (CF) has been linked to airway smooth muscle abnormalities including bronchial hyperresponsiveness. However, a role for CFTR in other types of smooth muscle, including myometrium, remains largely unexplored. As CF life expectancy and the number of pregnancies increases, there is a need for an understanding of the potential role of CFTR in myometrial function.<h4>Methods</h4>We investigated the role of CFTR in human and mouse myometrium. We used immunofluorescence to identify CFTR expression, and carried out contractility studies on spontaneously contracting term pregnant and non-pregnant mouse myometrium and term pregnant human myometrial biopsies from caesarean sections.<h4>Results</h4>CFTR was found to be expressed in term pregnant mouse myometrium. Inhibition of CFTR, with the selective inhibitor CFTR<sub>inh</sub>-172, significantly reduced contractility in pregnant mouse and human myometrium in a concentration-dependent manner (44.89 ± 11.02 term pregnant mouse, 9.23 ± 4.75 term-pregnant human; maximal effect at 60 μM expressed as a percentage of the pre-treatment control period). However, there was no effect of CFTR<sub>inh</sub>-172 in non-pregnant myometrium.<h4>Conclusion</h4>These results demonstrate decreased myometrial function when CFTR is inhibited, which may have implications on pregnancy and labour outcome and therapeutic decisions for labour in CF patients.

Item Type:	Article
Uncontrolled Keywords:	CFTR, Contractility, Cystic fibrosis, Human, Mouse, Myometrium, Pregnancy
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Depositing User:	Symplectic Admin
Date Deposited:	09 Apr 2024 09:17
Last Modified:	09 Apr 2024 13:31
DOI:	10.1016/j.crphys.2024.100122
Open Access URL:	https://doi.org/10.1016/j.crphys.2024.100122
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3180176