McCarron, Katy R, Elcocks, Hannah, Mortiboys, Heather, Urbé, Sylvie and Clague, Michael J 
ORCID: 0000-0003-3355-9479
(2024)
The Parkinson's disease related mutant VPS35 (D620N) amplifies the LRRK2 response to endolysosomal stress.
The Biochemical journal, 481 (4).
pp. 265-278.
Abstract
The identification of multiple genes linked to Parkinson's disease (PD) invites the question as to how they may co-operate. We have generated isogenic cell lines that inducibly express either wild-type or a mutant form of the retromer component VPS35 (D620N), which has been linked to PD. This has enabled us to test proposed effects of this mutation in a setting where the relative expression reflects the physiological occurrence. We confirm that this mutation compromises VPS35 association with the WASH complex, but find no defect in WASH recruitment to endosomes, nor in the distribution of lysosomal receptors, cation-independent mannose-6-phosphate receptor and Sortilin. We show VPS35 (D620N) enhances the activity of the Parkinson's associated kinase LRRK2 towards RAB12 under basal conditions. Furthermore, VPS35 (D620N) amplifies the LRRK2 response to endolysosomal stress resulting in enhanced phosphorylation of RABs 10 and 12. By comparing different types of endolysosomal stresses such as the ionophore nigericin and the membranolytic agent l-leucyl-l-leucine methyl ester, we are able to dissociate phospho-RAB accumulation from membrane rupture.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Endosomes, Lysosomes, Humans, Parkinson Disease, Vesicular Transport Proteins, Mutation, Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 09 Apr 2024 10:13 |
| Last Modified: | 09 Apr 2024 14:03 |
| DOI: | 10.1042/bcj20230492 |
| Open Access URL: | https://doi.org/10.1042/BCJ20230492 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3180226 |
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