Nanoengineering Carboxysome Shells for Protein Cages with Programmable Cargo Targeting.

Li, Tianpei, Chang, Ping, Chen, Weixian ORCID: 0009-0005-5402-9567, Shi, Zhaoyang, Xue, Chunling, Dykes, Gregory F, Huang, Fang, Wang, Qiang ORCID: 0000-0002-7388-4703 and Liu, Lu-Ning ORCID: 0000-0002-8884-4819 (2024) Nanoengineering Carboxysome Shells for Protein Cages with Programmable Cargo Targeting. ACS nano, 18 (10). pp. 7473-7484.

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Official URL: http://dx.doi.org/10.1021/acsnano.3c11559

Abstract

Protein nanocages have emerged as promising candidates for enzyme immobilization and cargo delivery in biotechnology and nanotechnology. Carboxysomes are natural proteinaceous organelles in cyanobacteria and proteobacteria and have exhibited great potential in creating versatile nanocages for a wide range of applications given their intrinsic characteristics of self-assembly, cargo encapsulation, permeability, and modularity. However, how to program intact carboxysome shells with specific docking sites for tunable and efficient cargo loading is a key question in the rational design and engineering of carboxysome-based nanostructures. Here, we generate a range of synthetically engineered nanocages with site-directed cargo loading based on an α-carboxysome shell in conjunction with SpyTag/SpyCatcher and Coiled-coil protein coupling systems. The systematic analysis demonstrates that the cargo-docking sites and capacities of the carboxysome shell-based protein nanocages could be precisely modulated by selecting specific anchoring systems and shell protein domains. Our study provides insights into the encapsulation principles of the α-carboxysome and establishes a solid foundation for the bioengineering and manipulation of nanostructures capable of capturing cargos and molecules with exceptional efficiency and programmability, thereby enabling applications in catalysis, delivery, and medicine.

Item Type:	Article
Uncontrolled Keywords:	Organelles, Bacterial Proteins, Biotechnology, Bioengineering, Protein Domains
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	10 Apr 2024 14:30
Last Modified:	10 Apr 2024 14:30
DOI:	10.1021/acsnano.3c11559
Open Access URL:	https://doi.org/10.1021/acsnano.3c11559
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3180266