Gerada, Alessandro 
ORCID: 0000-0002-6743-4271, Harper, Nicholas, Howard, Alex ORCID: 0000-0002-4195-6821, Reza, Nada ORCID: 0000-0003-1183-3262 and Hope, William ORCID: 0000-0001-6187-878X
(2024)
Determination of minimum inhibitory concentrations using machine-learning-assisted agar dilution.
Microbiology spectrum.
e0420923-e0420923.
Abstract
Effective policy to address the global threat of antimicrobial resistance requires robust antimicrobial susceptibility data. Traditional methods for measuring minimum inhibitory concentration (MIC) are resource intensive, subject to human error, and require considerable infrastructure. AIgarMIC streamlines and standardizes MIC measurement and is especially valuable for large-scale surveillance activities. MICs were measured using agar dilution for <i>n</i> = 10 antibiotics against clinical Enterobacterales isolates (<i>n</i> = 1,086) obtained from a large tertiary hospital microbiology laboratory. <i>Escherichia coli</i> (<i>n</i> = 827, 76%) was the most common organism. Photographs of agar plates were divided into smaller images covering one inoculation site. A labeled data set of colony images was created and used to train a convolutional neural network to classify images based on whether a bacterial colony was present (first-step model). If growth was present, a second-step model determined whether colony morphology suggested antimicrobial growth inhibition. The ability of the AI to determine MIC was then compared with standard visual determination. The first-step model classified bacterial growth as present/absent with 94.3% accuracy. The second-step model classified colonies as "inhibited" or "good growth" with 88.6% accuracy. For the determination of MIC, the rate of essential agreement was 98.9% (644/651), with a bias of -7.8%, compared with manual annotation. AIgarMIC uses artificial intelligence to automate endpoint assessments for agar dilution and potentially increases throughput without bespoke equipment. AIgarMIC reduces laboratory barriers to generating high-quality MIC data that can be used for large-scale surveillance programs.<h4>Importance</h4>This research uses modern artificial intelligence and machine-learning approaches to standardize and automate the interpretation of agar dilution minimum inhibitory concentration testing. Artificial intelligence is currently of significant topical interest to researchers and clinicians. In our manuscript, we demonstrate a use-case in the microbiology laboratory and present validation data for the model's performance against manual interpretation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | antimicrobial resistance, artificial intelligence, assay validation, digital health, image recognition, laboratory software, machine learning, minimum inhibitory concentration |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 16 Apr 2024 10:12 |
| Last Modified: | 16 Apr 2024 10:12 |
| DOI: | 10.1128/spectrum.04209-23 |
| Open Access URL: | https://doi.org/10.1128/spectrum.04209-23 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3180371 |
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