Bohns, Fabio Rocha, Akhtar, Riaz 
ORCID: 0000-0002-7963-6874, Chuang, Yung-Jen and Chen, Po-Yu
(2024)
Bone quality in zebrafish vertebrae improves after alendronate administration in a glucocorticoid-induced osteoporosis model.
Journal of the mechanical behavior of biomedical materials, 154.
p. 106521.
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Bohns et al JMBBM 2024.pdf - Author Accepted Manuscript Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
Glucocorticoid-induced osteoporosis (GIOP) changes the microarchitecture of bones and often leads to the reduction of bone-mineral density (BMD) and increased fracture rates. Zebrafish has been used as an alternative model for GIOP, however, the interaction of GIOP, and its treatment, with zebrafish bone morphometrics and mechanical properties, remains a challenge. Thus, this study aimed to evaluate the effects of prednisolone and alendronate on the properties of zebrafish vertebrae. Adult 7-month-old zebrafish were distributed into four groups: control (CTRL), prednisolone-only (PN), alendronate-only (ALN), and the sequential use of both medicines (PN + ALN). Fish skeletons were scanned via micro-tomography (n = 3) to obtain vertebra morphometrics (e.g., BMD). Bone morphology was assessed using scanning electron microscopy (n = 4) and the biomechanical behaviour with nanoindentation technique (n = 3). The BMD decreased in PN (426.08 ± 18.58 mg/cm<sup>3</sup>) and ALN (398.23 ± 10.20 mg/cm<sup>3</sup>) groups compared to the CTRL (490.43 ± 41.96 mg/cm<sup>3</sup>) (p < 0.001); however, administering the medicines in sequence recovered the values to healthy levels (495.43 ± 22.06 mg/cm<sup>3</sup>) (p > 0.05). The bone layered structures remain preserved in all groups. The vertebrae of the groups that received ALN and PN + ALN, displayed higher modulus of elasticity (27.27 ± 1.59 GPa and 25.68 ± 2.07 GPa, respectively) than the CTRL (22.74 ± 1.60 GP) (p < 0.001). ALN alone increased the hardness of zebrafish vertebrae to the highest value among the treatments (1.32 ± 0.13 GPa) (p < 0.001). Conversely, PN + ALN (1.25 ± 0.11 GPa) showed unaltered hardness from the CTRL (1.18 ± 0.13 GPa), but significantly higher than the PN group (1.08 ± 0.12 GPa) (p < 0.001). ALN administered after GIOP development, rescued osteoporotic condition by recovering the BMD and bone hardness in zebrafish vertebrae.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Spine, Lumbar Vertebrae, Animals, Zebrafish, Osteoporosis, Alendronate, Prednisolone, Glucocorticoids, Bone Density, Bone Density Conservation Agents |
| Divisions: | Faculty of Science and Engineering > School of Engineering |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 16 Apr 2024 10:35 |
| Last Modified: | 23 Apr 2024 13:20 |
| DOI: | 10.1016/j.jmbbm.2024.106521 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3180374 |
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