Identification of transporters involved in aromatic compounds tolerance through screening of transporter deletion libraries.

Sáez-Sáez, Javier, Munro, Lachlan Jake, Møller-Hansen, Iben, Kell, Douglas B ORCID: 0000-0001-5838-7963 and Borodina, Irina (2024) Identification of transporters involved in aromatic compounds tolerance through screening of transporter deletion libraries. Microb Biotechnol, 17 (4). e14460-e14460.

Access the full-text of this item by clicking on the Open Access link.

Abstract

Aromatic compounds are used in pharmaceutical, food, textile and other industries. Increased demand has sparked interest in exploring biotechnological approaches for their sustainable production as an alternative to chemical synthesis from petrochemicals or plant extraction. These aromatic products may be toxic to microorganisms, which complicates their production in cell factories. In this study, we analysed the toxicity of multiple aromatic compounds in common production hosts. Next, we screened a subset of toxic aromatics, namely 2-phenylethanol, 4-tyrosol, benzyl alcohol, berberine and vanillin, against transporter deletion libraries in Escherichia coli and Saccharomyces cerevisiae. We identified multiple transporter deletions that modulate the tolerance of the cells towards these compounds. Lastly, we engineered transporters responsible for 2-phenylethanol tolerance in yeast and showed improved 2-phenylethanol bioconversion from L-phenylalanine, with deletions of YIA6, PTR2 or MCH4 genes improving titre by 8-12% and specific yield by 38-57%. Our findings provide insights into transporters as targets for improving the production of aromatic compounds in microbial cell factories.

Item Type:	Article
Uncontrolled Keywords:	Escherichia coli, Saccharomyces cerevisiae, Organic Chemicals, Benzyl Alcohol, Phenylethyl Alcohol, Membrane Transport Proteins, Biotechnology
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	25 Apr 2024 10:09
Last Modified:	25 Apr 2024 12:11
DOI:	10.1111/1751-7915.14460
Open Access URL:	https://doi.org/10.1111/1751-7915.14460
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3180580