Liu, Jie, Garcia Bardales, Paul F, Islam, Kamrul, Jarju, Sheikh, Juma, Jane, Mhango, Chimwemwe, Naumanga, Queen, Qureshi, Sonia, Sonye, Catherine, Ahmed, Naveed et al (show 38 more authors)
(2024)
<i>Shigella</i> Detection and Molecular Serotyping With a Customized TaqMan Array Card in the Enterics for Global Health (EFGH): <i>Shigella</i> Surveillance Study.
Open forum infectious diseases, 11 (Suppl ).
S34-S40.
Abstract
<h4>Background</h4>Quantitative polymerase chain reaction (qPCR) targeting <i>ipaH</i> has been proven to be highly efficient in detecting <i>Shigella</i> in clinical samples compared to culture-based methods, which underestimate <i>Shigella</i> burden by 2- to 3-fold. qPCR assays have also been developed for <i>Shigella</i> speciation and serotyping, which is critical for both vaccine development and evaluation.<h4>Methods</h4>The Enterics for Global Health (EFGH) <i>Shigella</i> surveillance study will utilize a customized real-time PCR-based TaqMan Array Card (TAC) interrogating 82 targets, for the detection and differentiation of <i>Shigella</i> spp, <i>Shigella sonnei</i>, <i>Shigella flexneri</i> serotypes, other diarrhea-associated enteropathogens, and antimicrobial resistance (AMR) genes. Total nucleic acid will be extracted from rectal swabs or stool samples, and assayed on TAC. Quantitative analysis will be performed to determine the likely attribution of <i>Shigella</i> and other particular etiologies of diarrhea using the quantification cycle cutoffs derived from previous studies. The qPCR results will be compared to conventional culture, serotyping, and phenotypic susceptibility approaches in EFGH.<h4>Conclusions</h4>TAC enables simultaneous detection of diarrheal etiologies, the principal pathogen subtypes, and AMR genes. The high sensitivity of the assay enables more accurate estimation of <i>Shigella</i>-attributed disease burden, which is critical to informing policy and in the design of future clinical trials.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PCR, ipaH, quantification, rectal swab, serotyping |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 30 Apr 2024 08:07 |
| Last Modified: | 30 Apr 2024 08:08 |
| DOI: | 10.1093/ofid/ofad574 |
| Open Access URL: | https://doi.org/10.1093/ofid/ofad574 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3180643 |
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