Benedicto-Matambo, Prisca, Avolio, Lindsay N, Badji, Henry, Batool, Rabab, Khanam, Farhana, Munga, Stephen, Tapia, Milagritos D, Peñataro Yori, Pablo, Awuor, Alex O, Ceesay, Bubacarr E et al (show 37 more authors)
(2024)
Exploring Natural Immune Responses to <i>Shigella</i> Exposure Using Multiplex Bead Assays on Dried Blood Spots in High-Burden Countries: Protocol From a Multisite Diarrhea Surveillance Study.
Open forum infectious diseases, 11 (Suppl ).
S58-S64.
Abstract
<h4>Background</h4>Molecular diagnostics on human fecal samples have identified a larger burden of shigellosis than previously appreciated by culture. Evidence of fold changes in immunoglobulin G (IgG) to conserved and type-specific <i>Shigella</i> antigens could be used to validate the molecular assignment of type-specific <i>Shigella</i> as the etiology of acute diarrhea and support polymerase chain reaction (PCR)-based microbiologic end points for vaccine trials.<h4>Methods</h4>We will test dried blood spots collected at enrollment and 4 weeks later using bead-based immunoassays for IgG to invasion plasmid antigen B and type-specific lipopolysaccharide O-antigen for <i>Shigella flexneri</i> 1b, 2a, 3a, and 6 and <i>Shigella sonnei</i> in <i>Shigella</i>-positive cases and age-, site-, and season-matched test-negative controls from all sites in the Enterics for Global Health (EFGH) <i>Shigella</i> surveillance study. Fold antibody responses will be compared between culture-positive, culture-negative but PCR-attributable, and PCR-positive but not attributable cases and test-negative controls. Age- and site-specific seroprevalence distributions will be identified, and the association between baseline antibodies and <i>Shigella</i> attribution will be estimated.<h4>Conclusions</h4>The integration of these assays into the EFGH study will help support PCR-based attribution of acute diarrhea to type-specific <i>Shigella</i>, describe the baseline seroprevalence of conserved and type-specific <i>Shigella</i> antibodies, and support correlates of protection for immunity to <i>Shigella</i> diarrhea. These insights can help support the development and evaluation of <i>Shigella</i> vaccine candidates.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Shigella, diarrhea, dried blood spot, immune response, multiplex bead assay |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 30 Apr 2024 08:17 |
| Last Modified: | 30 Apr 2024 08:18 |
| DOI: | 10.1093/ofid/ofad650 |
| Open Access URL: | https://doi.org/10.1093/ofid/ofad650 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3180652 |
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