Microbiological Methods Used in the Enterics for Global Health <i>Shigella</i> Surveillance Study.

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Horne, Bri'Anna, Badji, Henry, Bhuiyan, Md Taufiqur Rahman, Romaina Cachique, Lucero, Cornick, Jennifer, Hotwani, Aneeta, Juma, Jane, Ochieng, John Benjamin, Abdou, Mahamadou, Apondi, Evans et al (show 39 more authors) , Atlas, Hannah E, Awuor, Alex O, Baker, Kate S ORCID: 0000-0001-5850-1949, Ceesay, Bubacarr E, Charles, Mary, Cunliffe, Nigel A ORCID: 0000-0002-5449-4988, Feutz, Erika, Galagan, Sean R, Guindo, Ibrehima, Hossain, M Jahangir, Iqbal, Junaid, Jallow, Fatima, Keita, Noumou Yakhouba, Khanam, Farhana, Kotloff, Karen L, Maiden, Victor, Manzanares Villanueva, Katia, Mito, Oscar, Mosharraf, Md Parvej, Nkeze, Joseph, Ikumapayi, Usman N, Paredes Olortegui, Maribel, Pavlinac, Patricia B, Pinedo Vasquez, Tackeshy, Qadri, Firdausi, Qamar, Farah Naz, Qureshi, Sonia, Rahman, Nazia, Sangare, Aminata, Sen, Sunil, Peñataro Yori, Pablo, Yousafzai, Mohammad Tahir ORCID: 0000-0001-8840-8323, Ahmed, Dilruba, Jere, Khuzwayo C ORCID: 0000-0003-3376-8529, Kosek, Margaret N, Omore, Richard, Permala-Booth, Jasnehta, Secka, Ousman and Tennant, Sharon M (2024) Microbiological Methods Used in the Enterics for Global Health <i>Shigella</i> Surveillance Study. Open forum infectious diseases, 11 (Suppl ). S25-S33.

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Abstract

<h4>Background</h4><i>Shigella</i> is a major cause of diarrhea in young children worldwide. Multiple vaccines targeting <i>Shigella</i> are in development, and phase 3 clinical trials are imminent to determine efficacy against shigellosis.<h4>Methods</h4>The Enterics for Global Health (EFGH) <i>Shigella</i> surveillance study is designed to determine the incidence of medically attended shigellosis in 6- to 35-month-old children in 7 resource-limited settings. Here, we describe the microbiological methods used to isolate and identify <i>Shigella</i>. We developed a standardized laboratory protocol for isolation and identification of <i>Shigella</i> by culture. This protocol was implemented across all 7 sites, ensuring consistency and comparability of results. Secondary objectives of the study are to determine the antibiotic resistance profiles of <i>Shigella</i>, compare isolation of <i>Shigella</i> from rectal swabs versus whole stool, and compare isolation of <i>Shigella</i> following transport of rectal swabs in Cary-Blair versus a modified buffered glycerol saline transport medium.<h4>Conclusions</h4>Data generated from EFGH using culture methods described herein can potentially be used for microbiological endpoints in future phase 3 clinical trials to evaluate vaccines against shigellosis and for other clinical and public health studies focused on these organisms.

Item Type:	Article
Uncontrolled Keywords:	Shigella, children, diarrhea, dysentery, microbiology
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	30 Apr 2024 08:25
Last Modified:	30 Apr 2024 08:25
DOI:	10.1093/ofid/ofad576
Open Access URL:	https://doi.org/10.1093/ofid/ofad576
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3180659