Imaging Isotopically Labeled Bacteria at the Single-Cell Level Using High-Resolution Optical Infrared Photothermal Spectroscopy.

Lima, Cassio, Muhamadali, Howbeer, Xu, Yun ORCID: 0000-0003-3228-5111, Kansiz, Mustafa and Goodacre, Royston ORCID: 0000-0003-2230-645X (2021) Imaging Isotopically Labeled Bacteria at the Single-Cell Level Using High-Resolution Optical Infrared Photothermal Spectroscopy. Analytical chemistry, 93 (6). pp. 3082-3088.

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Abstract

We report that the cellular uptake of stable isotope-labeled compounds by bacteria can be probed at the single-cell level using infrared spectroscopy, and this monitors the chemical vibrations affected by the incorporation of "heavy" atoms by cells and thus can be used to understand microbial systems. This presents a significant advancement as most studies have focused on evaluating communities of cells due to the poor spatial resolution achieved by classical infrared microspectrometers, and to date, there is no study evaluating the incorporation of labeled compounds by bacteria at single-cell levels using infrared spectroscopy. The development of new technologies and instrumentations that provide information on the metabolic activity of a single bacterium is critical as this will allow for a better understanding of the interactions between microorganisms as well as the function of individual members and their interactions in different microbial communities. Thus, the present study demonstrates the ability of a novel far-field infrared imaging technique, optical photothermal infrared (O-PTIR) spectroscopy, as a tool to monitor the uptake of ¹³C-glucose and ¹⁵N-ammonium chloride by <i>Escherichia coli</i> bacteria at single-cell levels using spectral signatures recorded via single-point and imaging modes. An additional novelty is that imaging was achieved using six vibrational bands in the amide I and II regions, which were analyzed with chemometrics by employing partial least squares-discriminant analysis to predict ¹³C/¹²C and ¹⁵N/¹⁴N simultaneously.

Item Type:	Article
Uncontrolled Keywords:	Bacteria, Diagnostic Imaging, Spectrophotometry, Infrared, Least-Squares Analysis, Microbiota
Depositing User:	Symplectic Admin
Date Deposited:	08 Feb 2021 16:41
Last Modified:	07 Feb 2023 15:26
DOI:	10.1021/acs.analchem.0c03967
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3115349